Journal: PLOS One

Article Title: Reappraisal of profibrotic phenotype and cell-cycle state of renal tubular epithelium after ischemia–reperfusion injury

doi: 10.1371/journal.pone.0347835

Figure Lengend Snippet: (A-E) RT-qPCR analysis showing temporal changes in gene expression. Vertical axis shows the relative expression difference (ΔΔCt value) for each mRNA normalized to glyceraldehyde 3-phosphate dehydrogenase. Values are expressed as fold change relative to the Stnd Cont group (set as 1.0). Time-course expression patterns are shown for (A) Ccn2 , Cellular Communication Network Factor 2, (B) Tgf-β , transforming growth factor-β, (C) Cdk1 , cyclin-dependent kinase 1, (D) Cdk6 , cyclin-dependent kinase 6, (E) p21 Waf1/Cip1 . The Wilcoxon test was used to compare the control group to the group with the greatest difference. * Statistically significant (p < 0.05) compared to the control. Abbreviations: RT-qPCR, RT-qPCR, real-time semi-quantitative reverse transcription polymerase chain reaction; HK-2, human kidney-2.

Article Snippet: The cultured human renal tubular epithelial cell line HK-2 (ATCC Catalog Number: CRL-2190) was cultured in the Ham’s F-12 and Dulbecco’s Modified Eagle Medium (4.5 g/L glucose) (Nacalai Tesque, Kyoto, Japan) supplemented with 5% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin in a humidified atmosphere at 37°C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Gene Expression, Expressing, Control, Reverse Transcription, Polymerase Chain Reaction